LbCas12a-intron
(Plasmid
#241900)
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PurposeLbCas12a-intron Gateway entry plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYPQ167
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLbCas12a-intron
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Insert Size (bp)5275
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Tags
/ Fusion Proteins
- NLS (C terminal on insert)
- NLS (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LbCas12a-intron was a gift from Yiping Qi (Addgene plasmid # 241900 ; http://n2t.net/addgene:241900 ; RRID:Addgene_241900) -
For your References section:
Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery. Luo W, Ors S, Fang H, Zeng M, Cheng Y, Sidawy AE, Qi Y. Plant Physiol. 2025 Nov 26;199(4):kiaf611. doi: 10.1093/plphys/kiaf611. 10.1093/plphys/kiaf611 PubMed 41335006