pFerCA
(Plasmid
#241903)
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PurposePart 1 of biosensor able to detect 13 substituted cinnamic acid based compounds
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonep15
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDepositor recommends growing plasmids in BL21 or DH10B chemically competent E. coli cells.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameferA and ferC
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SpeciesSynthetic
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Mutationnone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFerCA was a gift from Neil Dixon (Addgene plasmid # 241903 ; http://n2t.net/addgene:241903 ; RRID:Addgene_241903) -
For your References section:
Development and substrate specificity screening of an in vivo biosensor for the detection of biomass derived aromatic chemical building blocks. Machado LF, Dixon N. Chem Commun (Camb). 2016 Sep 15;52(76):11402-11405. doi: 10.1039/c6cc04559f. 10.1039/c6cc04559f PubMed 27722239