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Addgene

pLCeGFP
(Plasmid #241904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241904 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET44
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor recommends growing plasmids in BL21 or DH10B chemically competent E. coli cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    plc, His6-eGFP
  • Species
    Synthetic
  • Mutation
    none

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7 promoter replaced with LC promoter, uses native RNAP not T7 RNAP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCeGFP was a gift from Neil Dixon (Addgene plasmid # 241904 ; http://n2t.net/addgene:241904 ; RRID:Addgene_241904)

  • For your References section:

    Development and substrate specificity screening of an in vivo biosensor for the detection of biomass derived aromatic chemical building blocks. Machado LF, Dixon N. Chem Commun (Camb). 2016 Sep 15;52(76):11402-11405. doi: 10.1039/c6cc04559f. 10.1039/c6cc04559f PubMed 27722239
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