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Addgene

p44PPV-eGFP
(Plasmid #241907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241907 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET44
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor recommends growing plasmids in BL21 E. coli cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-eGFP
  • Species
    Synthetic
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p44PPV-eGFP was a gift from Neil Dixon (Addgene plasmid # 241907 ; http://n2t.net/addgene:241907 ; RRID:Addgene_241907)

  • For your References section:

    Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes. F M Machado L, Currin A, Dixon N. J Biol Eng. 2019 Nov 27;13:91. doi: 10.1186/s13036-019-0214-z. eCollection 2019. 10.1186/s13036-019-0214-z PubMed 31798685
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