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Addgene

NmCAT
(Plasmid #241909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241909 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTA231-p.Syn-PAmCherry1Hfx
  • Backbone manufacturer
    Anita Marchfelder (Addgene #164661)
  • Vector type
    Bacterial Expression
  • Selectable markers
    Novobiocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid can also be grown at 45 C, in NEB Stable cells, and in wild-type Haloferax mediterranei
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4HB-CoA synthetase
  • Species
    Nitrosopumilus maritimus
  • Mutation
    Mutated Haloferax gyrase B (gyrB*)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NmCAT was a gift from Neil Dixon (Addgene plasmid # 241909 ; http://n2t.net/addgene:241909 ; RRID:Addgene_241909)

  • For your References section:

    Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases. Zheng X, Chacón M, Allers T, Dixon N, Winterburn J. Biochem. Eng. J. 2024 Sep 16; 212. doi: 10.1016/j.bej.2024.109498. 10.1016/j.bej.2024.109498
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