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Addgene

p131CB10-tphKAB
(Plasmid #241911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241911 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSEVA131
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid can also be grown at 30 C, in NEB Stable cells, and in Pseudomonas putida KT2440. For growing in P. putida use ampicillin at 500 µg/ml.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tphK, tphA2, tphA3, tphB, tphA1
  • Species
    Synthetic
  • Mutation
    none

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p131CB10-tphKAB was a gift from Neil Dixon (Addgene plasmid # 241911 ; http://n2t.net/addgene:241911 ; RRID:Addgene_241911)

  • For your References section:

    Complex waste stream valorization through combined enzymatic hydrolysis and catabolic assimilation by Pseudomonas putida. Chacon M, Alvarez-Gonzalez G, Gosalvitr P, Berepiki A, Fisher K, Cuellar-Franca R, Dixon N. Trends Biotechnol. 2025 Mar;43(3):647-672. doi: 10.1016/j.tibtech.2024.10.020. Epub 2024 Dec 4. 10.1016/j.tibtech.2024.10.020 PubMed 39638703
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