pLOC-D133p53a
(Plasmid #241916)

• Purpose: Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.
• Depositing Lab: Curtis Harris
• Publication: Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. doi: 10.1016/j.neuroscience.2022.06.004. Epub 2022 Jun 15.

Backbone

• Vector backbone: pLOC-TurboRFP
• Backbone manufacturer: Open Biosystems (currently available from Horizon Discovery)
• Backbone size w/o insert (bp): 9264
• Total vector size (bp): 10050
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Delta133p53alpha
• Alt name: D133p53a
• Species: H. sapiens (human)
• Insert Size (bp): 786
• GenBank ID: NM_001126115.1
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: AscI (destroyed during cloning)
• 5′ sequencing primer: CMV-Forward
• 3′ sequencing primer: Internal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry