pAAV-CMV-HBA-RIBEYE-eGFP
(Plasmid
#241988)
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PurposeHuman RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV-GFP
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRIBEYE-GFP
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Alt nameRIBEYE/CtBP2
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SpeciesH. sapiens (human)
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Insert Size (bp)3705
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Entrez GeneCTBP2
- Promoter hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA)
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Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CCCACCTCTGCTGTATTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byReceived from Vladan Rankovic at the Institute for Auditory Neuroscience (University Medical Centre, Goettingen).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.7554/eLife.98254.1 for the ELife preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-HBA-RIBEYE-eGFP was a gift from Tobias Moser (Addgene plasmid # 241988 ; http://n2t.net/addgene:241988 ; RRID:Addgene_241988) -
For your References section:
Establishing synthetic ribbon-type active zones in a heterologous expression system. Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T. eLife13:RP98254 10.7554/eLife.98254.1