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f(syn)w-CMV-mKate2-p2A-RimBP2
(Plasmid #241989)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241989 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    f(syn)w-mKate2-P2A
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RIM-BP2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3267
  • GenBank ID
    NP_001074857.1
  • Entrez Gene
    Rimbp2 (a.k.a. A930033C01Rik, mKIAA0318)
  • Promoter human Synapsin
  • Tag / Fusion Protein
    • mKate2-p2A-RIMBP2 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Obtained from Maria Magdalena Picher who previously cloned the construct while at the Institute for Auditory Neuroscience, University Medical Centre, Göttingen

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.7554/eLife.98254.1 for the ELife preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    f(syn)w-CMV-mKate2-p2A-RimBP2 was a gift from Tobias Moser (Addgene plasmid # 241989 ; http://n2t.net/addgene:241989 ; RRID:Addgene_241989)
  • For your References section:

    Establishing synthetic ribbon-type active zones in a heterologous expression system. Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T. eLife13:RP98254 10.7554/eLife.98254.1