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pAAV-CMV-RBP2
(Plasmid #241990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241990 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-GFP
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RIM-BP2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3267
  • GenBank ID
    NP_001074857.1
  • Entrez Gene
    Rimbp2 (a.k.a. A930033C01Rik, mKIAA0318)
  • Promoter CMV enhancer
  • Tag / Fusion Protein
    • RIM-BP2 (untagged)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This untagged version for RBP2 expression was made by cloning the RBP2 cDNA obtained from f(syn)w-mKate2-p2A-RBP2 plasmid and replacing RIBEYE-GFP in the pAAV-CMV-HBA-RIBEYE-GFP-WPRE plasmid by in-fusion cloning (Takara Bio USA, Inc.).

Please visit https://doi.org/10.7554/eLife.98254.1 for the ELife preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-RBP2 was a gift from Tobias Moser (Addgene plasmid # 241990 ; http://n2t.net/addgene:241990 ; RRID:Addgene_241990)

  • For your References section:

    Establishing synthetic ribbon-type active zones in a heterologous expression system. Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T. eLife13:RP98254 10.7554/eLife.98254.1
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