pGGG-TaBSS1-tdTom
(Plasmid
#242014)
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PurposeL2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGoldenGreenGate-M
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Backbone manufacturerSmedley et al., 2021 Current Protocols
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato
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SpeciesTriticum aestivum
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Insert Size (bp)4359
- Promoter BSS1
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Tag
/ Fusion Protein
- NLS (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Position 2 insert described. Position 1 contains a hygromycin resistance cassette (hygromycin phosphotransferase II driven by PvUBI2).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGG-TaBSS1-tdTom was a gift from Cristobal Uauy (Addgene plasmid # 242014 ; http://n2t.net/addgene:242014 ; RRID:Addgene_242014)