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pCSII-cp PS Intein tTA Puro
(Plasmid #242027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242027 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCSII-EF-MCS
  • Backbone manufacturer
    Dr. Hiroyuki Miyoshi (RIKEN, Tsukuba, Japan)
  • Backbone size w/o insert (bp) 8475
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cp PS Intein tTA Puro
  • Alt name
    ERT2-Linker-IntC1-Linker 1-VVD-Linker 2-VVD-Linker 3-IntC2-SSS-tTA-Linker-SGY-IntN-Linker-ERT2
  • Species
    Synthetic
  • Insert Size (bp)
    5157
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSII-cp PS Intein tTA Puro was a gift from Vladislav Verkhusha (Addgene plasmid # 242027 ; http://n2t.net/addgene:242027 ; RRID:Addgene_242027)

  • For your References section:

    Photoswitchable intein for light control of covalent protein binding and cleavage. Baloban M, Manoilov KY, Karasev MM, Verkhusha VV, Shcherbakova DM. Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9. 10.1038/s41467-025-63595-9 PubMed 40935816
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