phTERT-Split PS Intein-N tTA
(Plasmid #242034)

• Purpose: N_terminal segment of the blue_light_controlled split PS_intein tTA gene expression system, co_expressing mTagBFP2 under the hTERT promoter.
• Depositing Lab: Vladislav Verkhusha
• Publication: Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9.

Backbone

• Vector backbone: mEGFP-N1
• Backbone size w/o insert (bp): 5041
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Split PS Intein-N tTA
• Alt name: M-SGY-IntN--T2A-mTagBFP2
• Species: Synthetic
• Insert Size (bp): 1047
• Mutation: The CMV promoter was replaced with the hTERT promoter, which was amplified from the genome of HeLa cells, in the original vector.
• Promoter: hTERT
• Tag / Fusion Protein:
  • mTagBFP2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pBR322ori-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry