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pNF-KB-Split PS Intein-C tTA
(Plasmid #242035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242035 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    mEGFP-N1
  • Backbone size w/o insert (bp) 3628
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Split PS Intein-C tTA
  • Alt name
    IntC1-Linker 1-VVD-Linker 2-VVD-Linker 3-IntC-SSS-tTA--T2A-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    3868
  • Mutation
    The CMV promoter in the original vector was replaced with the NF-KB promoter, which was amplified from the pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro] vector.
  • Promoter NF-KB
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNF-KB-Split PS Intein-C tTA was a gift from Vladislav Verkhusha (Addgene plasmid # 242035 ; http://n2t.net/addgene:242035 ; RRID:Addgene_242035)

  • For your References section:

    Photoswitchable intein for light control of covalent protein binding and cleavage. Baloban M, Manoilov KY, Karasev MM, Verkhusha VV, Shcherbakova DM. Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9. 10.1038/s41467-025-63595-9 PubMed 40935816
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