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Addgene

pNQ078
(Plasmid #242083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242083 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pVS10
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpoA-rpoB-rpoC-Cter-ybbR-6xHis and rpoZ
  • Species
    E. coli
  • Mutation
    ybbR-peptide tag inserted between E1377 and G1402, thereby deleting the region A1378–L1401 in rpoC
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNQ078 was a gift from Olivier Duss (Addgene plasmid # 242083 ; http://n2t.net/addgene:242083 ; RRID:Addgene_242083)

  • For your References section:

    Tracking transcription-translation coupling in real time. Qureshi NS, Duss O. Nature. 2025 Jan;637(8045):487-495. doi: 10.1038/s41586-024-08308-w. Epub 2024 Dec 4. 10.1038/s41586-024-08308-w PubMed 39633055
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