SvPchE
(Plasmid
#242093)
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PurposeNonribosomal peptide synthetase from Streptomyces venezuluelae for the production of watasemysin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET29b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSvPchE
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SpeciesStreptomyces venezuelae
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Insert Size (bp)4599
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GenBank IDWP_306327423.1
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Tag
/ Fusion Protein
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SvPchE was a gift from Audrey Lamb (Addgene plasmid # 242093 ; http://n2t.net/addgene:242093 ; RRID:Addgene_242093) -
For your References section:
Stuffed Epimerase Domains of Pyochelin Biosynthesis are Defunct Methyltransferases. Ronnebaum TA, Meneely KM, Horsman GP, Prisinzano TE, Lamb AL. Biochemistry. 2025 Sep 16;64(18):3919-3934. doi: 10.1021/acs.biochem.5c00172. Epub 2025 Aug 26. 10.1021/acs.biochem.5c00172 PubMed 40857142