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PaPchE-AE
(Plasmid #242103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242103 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PaPchE-AE
  • Species
    Pseudomonas aeruginosa
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PaPchE-AE was a gift from Audrey Lamb (Addgene plasmid # 242103 ; http://n2t.net/addgene:242103 ; RRID:Addgene_242103)

  • For your References section:

    Stuffed Epimerase Domains of Pyochelin Biosynthesis are Defunct Methyltransferases. Ronnebaum TA, Meneely KM, Horsman GP, Prisinzano TE, Lamb AL. Biochemistry. 2025 Sep 16;64(18):3919-3934. doi: 10.1021/acs.biochem.5c00172. Epub 2025 Aug 26. 10.1021/acs.biochem.5c00172 PubMed 40857142
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