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PaPchF-AMT
(Plasmid #242104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242104 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PaPchF-AMT
  • Species
    Pseudomonas aeruginosa
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PaPchF-AMT was a gift from Audrey Lamb (Addgene plasmid # 242104 ; http://n2t.net/addgene:242104 ; RRID:Addgene_242104)

  • For your References section:

    Stuffed Methyltransferase Catalyzes the Penultimate Step of Pyochelin Biosynthesis. Ronnebaum TA, McFarlane JS, Prisinzano TE, Booker SJ, Lamb AL. Biochemistry. 2019 Feb 12;58(6):665-678. doi: 10.1021/acs.biochem.8b00716. Epub 2018 Dec 27. 10.1021/acs.biochem.8b00716 PubMed 30525512
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