pKC107_pLenti_TetON_sfGFP_INT
(Plasmid
#242109)
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PurposeTet- or Dox-inducible expression of sfGFP fused to INT (human PARP4 fragment)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti-tetON
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARP4 fragment
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Alt namesfGFP_INT
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SpeciesSynthetic
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MutationPARP4 aa 1562-1724
- Promoter TRE3G
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Tag
/ Fusion Protein
- sfGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes TagRFP-Tet-On3G
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKC107_pLenti_TetON_sfGFP_INT was a gift from Fei Chen (Addgene plasmid # 242109 ; http://n2t.net/addgene:242109 ; RRID:Addgene_242109)