pKC105_pLenti_TetON_PABPC1_INT
(Plasmid
#242110)
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PurposeTet- or Dox-inducible expression of PABPC1 fused to INT (human PARP4 fragment)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti-tetON
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAB4L, PARP4 fragment
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Alt namePABPC1_INT
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SpeciesH. sapiens (human)
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MutationPABPC1 aa 1-370, PARP4 aa 1562-1724
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Entrez GenePABPC1 (a.k.a. PAB1, PABP, PABP1, PABPC2, PABPL1)
- Promoter TRE3G
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes TagRFP-Tet-On3G
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKC105_pLenti_TetON_PABPC1_INT was a gift from Fei Chen (Addgene plasmid # 242110 ; http://n2t.net/addgene:242110 ; RRID:Addgene_242110)