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Addgene

pcDNA-DEST47-SIRT7
(Plasmid #242137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242137 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA-DEST47
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7780
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    SIRT7 (a.k.a. SIR2L7)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cycle 3 GFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-DEST47-SIRT7 was a gift from J. Carl Barrett & Izumi Horikawa (Addgene plasmid # 242137 ; http://n2t.net/addgene:242137 ; RRID:Addgene_242137)

  • For your References section:

    Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Michishita E, Park JY, Burneskis JM, Barrett JC, Horikawa I. Mol Biol Cell. 2005 Oct;16(10):4623-35. doi: 10.1091/mbc.e05-01-0033. Epub 2005 Aug 3. 10.1091/mbc.e05-01-0033 PubMed 16079181
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