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Addgene

pLlacO1-DP6
(Plasmid #242138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242138 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    DP6
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Plasmid requires a bacterial strain overexpressing LacI (LacIq) to propagate (such as NEB Stable or XL1-Blue), as the plasmid does not contain this protein and cassette will be toxic otherwise.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gene III
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on the design of PLlac-MP6-SpecR (Brödel et. al 2020, https://doi.org/10.1126/sciadv.aba2728) with DP6 as the backbone.

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLlacO1-DP6 was a gift from Dominik Niopek (Addgene plasmid # 242138 ; http://n2t.net/addgene:242138 ; RRID:Addgene_242138)
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