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PACE_SP_SD4_AraC_R5_S170_LOV
(Plasmid #242146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242146 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    SPentry_Kan_ccdB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AraC R5 S170 LOV
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Designed to assemble into an AraC R5 S170 LOV containing, Gene III Deficient M13 phage upon SapI golden gate cloning with vectors pBT114 (Addgene plasmid #138523) and pBT29 (Addgene plasmid #122599) and transformation into S2208.

Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PACE_SP_SD4_AraC_R5_S170_LOV was a gift from Dominik Niopek (Addgene plasmid # 242146 ; http://n2t.net/addgene:242146 ; RRID:Addgene_242146)
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