pDest_PANCEd_AraC_sd2_R2_C1
(Plasmid
#242149)
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PurposeExpression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDest
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAraC R2
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SpeciesSynthetic
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MutationE3K, R146L, A152T, R251L
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest_PANCEd_AraC_sd2_R2_C1 was a gift from Dominik Niopek (Addgene plasmid # 242149 ; http://n2t.net/addgene:242149 ; RRID:Addgene_242149)