RcP9
(Plasmid
#242157)
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Purpose(Empty Backbone) Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneDP6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains CcdB Dropout Cassette. Take care to grow on a resistant strain for plasmid amplification. For ease of use when constructing oligo libraries for RAMPhaGE Libraries, please find the corresponding RAMPhaGE Oligo Tool Colab Notebook: https://colab.research.google.com/github/Niopek-Lab/POGO_PANCE/blob/main/scripts/RAMPhaGE_Oligo_Tool.ipynb.
Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RcP9 was a gift from Dominik Niopek (Addgene plasmid # 242157 ; http://n2t.net/addgene:242157 ; RRID:Addgene_242157)
