RcP9_AraC_S170_LOV_P190_Sil_90bp
(Plasmid
#242158)
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PurposeRecombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneRcP9
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGene III
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RcP9_AraC_S170_LOV_P190_Sil_90bp was a gift from Dominik Niopek (Addgene plasmid # 242158 ; http://n2t.net/addgene:242158 ; RRID:Addgene_242158)