CMDuoRed_Crop-Seq-H2B-mCherry-WPRE-TS-hU6-BsmbI
(Plasmid
#242160)
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Purpose(Empty Backbone) Empty lentiviral backbone for generation of ClonMapper Duo Red (H2B-mCherry) gRNA barcode library for use with polyA capture or gRNA capture scRNA-seq technologies
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneCROPseq-Guide-Puro
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://docs.brocklab.com/clonmapper for an updated use protocol. Please visit https://doi.org/10.1101/2024.12.11.627873 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMDuoRed_Crop-Seq-H2B-mCherry-WPRE-TS-hU6-BsmbI was a gift from Amy Brock (Addgene plasmid # 242160 ; http://n2t.net/addgene:242160 ; RRID:Addgene_242160)
