lenti-EF1a-dCas9-KRAB-H2B-GFP
(Plasmid
#242166)
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PurposeLentiviral expression of dCas9-KRAB with H2B-GFP reporter
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonelenti-EF1a-dCas9-KRAB-Puro
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Backbone manufacturerKristen Brennand (AddGene #99372)
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-KRAB; T2A-H2B-GFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti-EF1a-dCas9-KRAB-H2B-GFP was a gift from Amy Brock (Addgene plasmid # 242166 ; http://n2t.net/addgene:242166 ; RRID:Addgene_242166)