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XLone-Fully-spliced CRNDE
(Plasmid #242178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242178 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    XLone-tTA-MCS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fully-spliced CRNDE
  • Species
    H. sapiens (human)
  • Mutation
    PAM mutation (CCT to GTT)
  • Entrez Gene
    CRNDE (a.k.a. CRNDEP, LINC00180, NCRNA00180, PNAS-108, lincIRX5)
  • Promoter TRE3GS

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We observed two small deletions (6 base pair and 14 base pair) within the CRNDE insert, however, the expression of the fully spliced transcript is expected to be retained.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-Fully-spliced CRNDE was a gift from Joshua Mendell (Addgene plasmid # 242178 ; http://n2t.net/addgene:242178 ; RRID:Addgene_242178)

  • For your References section:

    An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation. Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT. Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3. 10.1016/j.molcel.2025.03.006 PubMed 40185099
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