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Addgene

XLone-UCE intron
(Plasmid #242180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242180 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    XLone-tTA-MCS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRNDE UCE intron
  • Species
    H. sapiens (human)
  • Entrez Gene
    CRNDE (a.k.a. CRNDEP, LINC00180, NCRNA00180, PNAS-108, lincIRX5)
  • Promoter TRE3GS

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-UCE intron was a gift from Joshua Mendell (Addgene plasmid # 242180 ; http://n2t.net/addgene:242180 ; RRID:Addgene_242180)

  • For your References section:

    An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation. Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT. Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3. 10.1016/j.molcel.2025.03.006 PubMed 40185099
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