Skip to main content
Addgene

hAAVS-RPL23a-SNAP
(Plasmid #242187)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242187 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    hAAVS1-HYG-CMV-EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPL23a
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPL23A (a.k.a. MDA20, FLJ27455)
  • Promoter CMV
  • Tag / Fusion Protein
    • SNAP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

~92 bp insertion in the 3′ UTR between SnapTag stop and SV40 poly(A); coding sequence unchanged.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hAAVS-RPL23a-SNAP was a gift from Joshua Mendell (Addgene plasmid # 242187 ; http://n2t.net/addgene:242187 ; RRID:Addgene_242187)

  • For your References section:

    An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation. Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT. Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3. 10.1016/j.molcel.2025.03.006 PubMed 40185099
Related items: