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pLenti-eIF6-3XFlag
(Plasmid #242188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242188 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Lenti-CMV-Blast
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF6
  • Species
    H. sapiens (human)
  • Entrez Gene
    EIF6 (a.k.a. CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27BBP)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

~51 bp deletion at the CMV-insert junction (Kozak/ATG preserved) and ~282 bp deletion in the TEV/linker region upstream of FLAG; eIF6-FLAG remains in-frame and expresses; TEV function may be altered.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-eIF6-3XFlag was a gift from Joshua Mendell (Addgene plasmid # 242188 ; http://n2t.net/addgene:242188 ; RRID:Addgene_242188)

  • For your References section:

    An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation. Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT. Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3. 10.1016/j.molcel.2025.03.006 PubMed 40185099
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