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pGMF2-M
(Plasmid #242204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242204 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUCIDT-AMP
  • Backbone manufacturer
    IDT
  • Total vector size (bp) 4101
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
  • gRNA/shRNA sequence
    n/a
  • Species
    Synthetic
  • Promoter OsU6-2p

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer CTGGTGGCAGGATATATTGTGGTG
  • 3′ sequencing primer GAACCCTGTGGTTGGCATGCACATAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMF2-M was a gift from Kate Creasey Krainer (Addgene plasmid # 242204 ; http://n2t.net/addgene:242204 ; RRID:Addgene_242204)

  • For your References section:

    Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta. Abate BA, Hahn F, Chirivì D, Betti C, Fornara F, Molloy MC, Creasey Krainer KM. bioRxiv November 9, 2025 10.1101/2025.11.09.687425
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