pGMF3
(Plasmid
#242207)
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Purpose(Empty Backbone) L2 binary T-DNA backbone, suitable for plant transformation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepICSL4723
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAdapted from pICSL4723 (Mark Youles, the Sainsbury Lab)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
More information on https://www.growmorefoundation.org/enable-crispr-kits
Please note: This plasmid contains a C nucleotide deletion at P302 in dxs E.coli causing a frameshift and early termination. This deletion does not impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMF3 was a gift from Kate Creasey Krainer (Addgene plasmid # 242207 ; http://n2t.net/addgene:242207 ; RRID:Addgene_242207) -
For your References section:
Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta. Abate BA, Hahn F, Chirivì D, Betti C, Fornara F, Molloy MC, Creasey Krainer KM. bioRxiv November 9, 2025 10.1101/2025.11.09.687425