pGMF6
(Plasmid
#242208)
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Purpose35Sp::eGFP:35St cassette in L1 vector backbone. Expresses a nuclear localized eGFP protein for transient plant transformation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUCIDT-AMP
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Backbone manufacturerIDT
- Total vector size (bp) 5780
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLevel1 35Sp::eGFP:35St
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMF6 was a gift from Kate Creasey Krainer (Addgene plasmid # 242208 ; http://n2t.net/addgene:242208 ; RRID:Addgene_242208) -
For your References section:
Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta. Abate BA, Hahn F, Chirivì D, Betti C, Fornara F, Molloy MC, Creasey Krainer KM. bioRxiv November 9, 2025 10.1101/2025.11.09.687425