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Addgene

pRRL-CAG-NR5A1-FLAG
(Plasmid #242229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242229 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRRL
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NR5A1
  • Alt name
    SF-1
  • Species
    H. sapiens (human)
  • Entrez Gene
    NR5A1 (a.k.a. AD4BP, ELP, FTZ1, FTZF1, POF7, SF-1, SF1, SPGF8, SRXX4, SRXY3, hSF-1)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL-CAG-NR5A1-FLAG was a gift from Courtney Hodges (Addgene plasmid # 242229 ; http://n2t.net/addgene:242229 ; RRID:Addgene_242229)

  • For your References section:

    beta-catenin functions as a molecular adapter for disordered cBAF interactions. Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, Cermakova K, Hodges HC. Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 10.1016/j.molcel.2025.06.026. 10.1016/j.molcel.2025.06.026 PubMed 40695292
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