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pAKanCRISPR
(Plasmid #242231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242231 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACRISPR
  • Backbone manufacturer
    This was a modification of the plasmid pACRISPR (Addgene plasmid #113348) from Quanjiang Ji.
  • Backbone size (bp) 5969
  • Modifications to backbone
    Ampicillin resistance gene (AmpR) was replaced with Kanamycin resistance gene.
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKanCRISPR was a gift from James T. Tambong (Addgene plasmid # 242231 ; http://n2t.net/addgene:242231 ; RRID:Addgene_242231)

  • For your References section:

    Phenazines are involved in the antagonism of a novel subspecies of Pseudomonas chlororaphis strain S1Bt23 against Pythium ultimum. Chi SI, Akuma M, Xu R, Plante V, Hadinezhad M, Tambong JT. Sci Rep. 2024 Sep 3;14(1):20517. doi: 10.1038/s41598-024-71418-y. 10.1038/s41598-024-71418-y PubMed 39227476
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