pAKanCRISPR
(Plasmid
#242231)
-
Purpose(Empty Backbone) A sgRNA expression plasmid for genome editing in Pseudomonas
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepACRISPR
-
Backbone manufacturerThis was a modification of the plasmid pACRISPR (Addgene plasmid #113348) from Quanjiang Ji.
- Backbone size (bp) 5969
-
Modifications to backboneAmpicillin resistance gene (AmpR) was replaced with Kanamycin resistance gene.
-
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAKanCRISPR was a gift from James T. Tambong (Addgene plasmid # 242231 ; http://n2t.net/addgene:242231 ; RRID:Addgene_242231)