pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato
(Plasmid
#242233)
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PurposeExpress Kir 2.1 and dTomato in the neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonePAAV-Syn
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Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameFas
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Insert Size (bp)34
- Promoter SYN
Cloning Information for Gene/Insert 1
- Cloning method Gene Synthesis
- 5′ sequencing primer aacttcgtatatacc (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameMyc/Kir2.1 (E224GY242F)
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SpeciesSynthetic
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Insert Size (bp)1314
- Promoter SYN
Cloning Information for Gene/Insert 2
- Cloning method Gene Synthesis
- 5′ sequencing primer agaagctgatcagcgaggagg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato was a gift from Qingchun Tong (Addgene plasmid # 242233 ; http://n2t.net/addgene:242233 ; RRID:Addgene_242233) -
For your References section:
An alternative neural basis underlying leptin resistance. Li H, Su C, Xu Y, Ludwig MQ, Davis J, Tong Q. Cell Rep. 2025 Jun 19;44(7):115863. doi: 10.1016/j.celrep.2025.115863. 10.1016/j.celrep.2025.115863 PubMed 40540394