pET21a asyn(Δ103-130)
(Plasmid
#242235)
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PurposeExpresses alpha synuclein (missing exon 5, residues 103-130) in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET21a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameasyn(Δ103-130)
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SpeciesH. sapiens (human)
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Mutationdeletion of residues 103-130
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Tag
/ Fusion Protein
- none
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a asyn(Δ103-130) was a gift from Jennifer Lee (Addgene plasmid # 242235 ; http://n2t.net/addgene:242235 ; RRID:Addgene_242235) -
For your References section:
Amyloid formation of alternatively spliced variants of alpha-synuclein. SanGiovanni DQ, McGlinchey RP, Lee JC. Protein Sci. 2025 Jul;34(7):e70195. doi: 10.1002/pro.70195. 10.1002/pro.70195 PubMed 40522183