hPDGFA-EGFP
(Plasmid
#242343)
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Purposemammalian expression construct for human Platelet-derived growth factor subunit A C-terminally tagged with EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlatelet-derived growth factor subunit A
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Alt namePDGFA
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SpeciesH. sapiens (human)
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Insert Size (bp)633
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Entrez GenePDGFA (a.k.a. PDGF-A, PDGF1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hPDGFA-EGFP was a gift from Christoph Kaether (Addgene plasmid # 242343 ; http://n2t.net/addgene:242343 ; RRID:Addgene_242343)