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pCDNA3-APOX
(Plasmid #242344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242344 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5388
  • Total vector size (bp) 6159
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLAG-APOX
  • Alt name
    APEX2 (F41V-S69I-Y235F-H239Y)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    771
  • Mutation
    F41V, S69I, Y235F, H239Y
  • Entrez Gene
    APEX2 (a.k.a. APE2, APEXL2, XTH2, ZGRF2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer taatacgactcactataggg
  • 3′ sequencing primer aacagatggctggcaac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.26434/chemrxiv-2025-07zmn for the ChemRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-APOX was a gift from Jeffrey Martell (Addgene plasmid # 242344 ; http://n2t.net/addgene:242344 ; RRID:Addgene_242344)

  • For your References section:

    Directed Evolution of APOX for Proximity Labeling Using Phenols with High Redox Potentials. Fang S, Acevedo LD, Solivais AJ, Zhou X, Delfosse ES, Frey BL, Smith LM, Martell JD. ChemRxiv. 2025; doi:10.26434/chemrxiv-2025-07zmn 10.26434/chemrxiv-2025-07zmn
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