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pCMV CHMP2Bchimera-L4D,F5D-CHMP6α2-3XHA
(Plasmid #242419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242419 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHM6
  • Backbone size w/o insert (bp) 5450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CHMP2B
  • Species
    H. sapiens (human)
  • Mutation
    L4D F5D; Helix 2 (residues 55-96) replaced with residues 57-98 from CHMP6
  • Entrez Gene
    CHMP2B (a.k.a. ALS17, CHMP2.5, DMT1, FTDALS7, VPS2-2, VPS2B)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CHMP6
  • Species
    H. sapiens (human)
  • Mutation
    Residues 57-98
  • Entrez Gene
    CHMP6 (a.k.a. VPS20)
  • Promoter CMV

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.13.632710 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV CHMP2Bchimera-L4D,F5D-CHMP6α2-3XHA was a gift from Franz-Ulrich Hartl (Addgene plasmid # 242419 ; http://n2t.net/addgene:242419 ; RRID:Addgene_242419)

  • For your References section:

    alpha-Synuclein aggregates inhibit ESCRT-III through sequestration and collateral degradation. Sitron CS, Trinkaus VA, Galesic A, Garhammer M, Yuste-Checa P, Dransfeld U, Feigenbutz D, Zhang J, Ivashko L, Dudanova I, Harper JW, Hartl FU. Mol Cell. 2025 Sep 18;85(18):3505-3523.e17. doi: 10.1016/j.molcel.2025.08.022. Epub 2025 Sep 10. 10.1016/j.molcel.2025.08.022 PubMed 40934925
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