pCMV sfGFP-CHMP6 57-98
(Plasmid
#242420)
-
PurposeExpression of the second alpha helix of CHMP6 (residues 57-98), N-terminally tagged with sfGFP
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242420 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepHM6
- Backbone size w/o insert (bp) 5450
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCHMP6
-
SpeciesH. sapiens (human)
-
MutationResidues 57-98
-
Entrez GeneCHMP6 (a.k.a. VPS20)
- Promoter CMV
-
Tag
/ Fusion Protein
- sfGFP (superfolder GFP) (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.01.13.632710 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV sfGFP-CHMP6 57-98 was a gift from Franz-Ulrich Hartl (Addgene plasmid # 242420 ; http://n2t.net/addgene:242420 ; RRID:Addgene_242420)