pH-SpRY-PCE
(Plasmid
#242428)
-
Purposefor chromosome editing
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepHUE411
-
Vector typePlant Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)EPI300
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepGSPE-epeg, SpRY-PE, cm24Cre
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pH-SpRY-PCE was a gift from Caixia Gao (Addgene plasmid # 242428 ; http://n2t.net/addgene:242428 ; RRID:Addgene_242428) -
For your References section:
Iterative recombinase technologies for efficient and precise genome engineering across kilobase to megabase scales. Sun C, Li H, Liu Y, Li Y, Gao R, Shi X, Fei H, Liu J, Liang R, Gao C. Cell. 2025 Aug 1:S0092-8674(25)00800-1. doi: 10.1016/j.cell.2025.07.011. 10.1016/j.cell.2025.07.011 PubMed 40763736