pVBL0001
(Plasmid
#242429)
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PurposeStable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepcDNA5
- Backbone size w/o insert (bp) 5182
- Total vector size (bp) 9130
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRE1alpha
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Alt nameIRE1a
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Alt nameERN1
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SpeciesH. sapiens (human)
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Insert Size (bp)3948
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MutationDeletion of the lumenal domain, addition of two C-terminal fusions (mCherry and CRY2clust)
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GenBank IDNM_001433.5
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Entrez GeneERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
- Promoter CMV (tet-on, inducible)
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- CRY2clust (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer multiple primers (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVBL0001 was a gift from Vladislav Belyy (Addgene plasmid # 242429 ; http://n2t.net/addgene:242429 ; RRID:Addgene_242429) -
For your References section:
Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPR. Smith JW, Wilburn DB, Belyy V. bioRxiv [Preprint]. 2025 Jul 21:2025.07.16.665212. doi: 10.1101/2025.07.16.665212. 10.1101/2025.07.16.665212 PubMed 40777275