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Addgene

pVBL0001
(Plasmid #242429)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242429 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pcDNA5
  • Backbone size w/o insert (bp) 5182
  • Total vector size (bp) 9130
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRE1alpha
  • Alt name
    IRE1a
  • Alt name
    ERN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3948
  • Mutation
    Deletion of the lumenal domain, addition of two C-terminal fusions (mCherry and CRY2clust)
  • GenBank ID
    NM_001433.5
  • Entrez Gene
    ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
  • Promoter CMV (tet-on, inducible)
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • CRY2clust (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVBL0001 was a gift from Vladislav Belyy (Addgene plasmid # 242429 ; http://n2t.net/addgene:242429 ; RRID:Addgene_242429)
  • For your References section:

    Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPR. Smith JW, Wilburn DB, Belyy V. bioRxiv [Preprint]. 2025 Jul 21:2025.07.16.665212. doi: 10.1101/2025.07.16.665212. 10.1101/2025.07.16.665212 PubMed 40777275