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Addgene

pUC19_21 tRNA genes
(Plasmid #242430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242430 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    GenScript
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
  • Species
    Escherichia coli
  • Insert Size (bp)
    2305

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_21 tRNA genes was a gift from Sebastian Maerkl (Addgene plasmid # 242430 ; http://n2t.net/addgene:242430 ; RRID:Addgene_242430)

  • For your References section:

    Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system. Li F, Baranwal AK, Maerkl SJ. Nat Commun. 2025 Jul 5;16(1):6212. doi: 10.1038/s41467-025-61671-8. 10.1038/s41467-025-61671-8 PubMed 40617857
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