pERV3-zeo
(Plasmid
#242432)
-
PurposeThe pERV3 vector expresses both VgEcR and RXR from a dicistronic mRNA transcribed under the control of the CMV promoter. This is achieved by inserting an internal ribosome entry site (IRES) between th
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepHAGE
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersZeocin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameVgEcR and RXR
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AAATGGGCGGTAGGCGTG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pERV3-zeo was a gift from Hanae Sato & Robert Singer (Addgene plasmid # 242432 ; http://n2t.net/addgene:242432 ; RRID:Addgene_242432) -
For your References section:
Cellular variability of nonsense-mediated mRNA decay. Sato H, Singer RH. Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0. 10.1038/s41467-021-27423-0 PubMed 34893608
