pCMV-TO-CasRx(NLS)-T2A-mCherry
(Plasmid
#242433)
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PurposeCasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSMART HC_Amp
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx
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Tag
/ Fusion Protein
- Tag / Fusion Protein: T2A mCherry
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-TO-CasRx(NLS)-T2A-mCherry was a gift from Diego Di Bernardo (Addgene plasmid # 242433 ; http://n2t.net/addgene:242433 ; RRID:Addgene_242433) -
For your References section:
Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems. De Carluccio G, Fusco V, di Bernardo D. Nat Commun. 2024 Apr 17;15(1):3311. doi: 10.1038/s41467-024-47592-y. 10.1038/s41467-024-47592-y PubMed 38632224