pCMV-gLuc-DR_pCMV-RedFireFly.gb
(Plasmid
#242435)
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PurposeGaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSMART HC_Amp
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Vector typeCRISPR, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGaussia Luciferase & Red Firefly Luciferase
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains a 134bp deletion and 90bp insertion in the backbone that removes SV40 ori from the plasmid. This change does not impact the plasmid's function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-gLuc-DR_pCMV-RedFireFly.gb was a gift from Diego Di Bernardo (Addgene plasmid # 242435 ; http://n2t.net/addgene:242435 ; RRID:Addgene_242435) -
For your References section:
Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems. De Carluccio G, Fusco V, di Bernardo D. Nat Commun. 2024 Apr 17;15(1):3311. doi: 10.1038/s41467-024-47592-y. 10.1038/s41467-024-47592-y PubMed 38632224
Map uploaded by the depositor.
