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Addgene

pET-15b_T. maritima His-tRNase Z
(Plasmid #242436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242436 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    GenScript
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNase Z
  • Species
    Thermotoga maritima
  • Insert Size (bp)
    870
  • GenBank ID
    NP_228673
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer T7 forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b_T. maritima His-tRNase Z was a gift from Sebastian Maerkl (Addgene plasmid # 242436 ; http://n2t.net/addgene:242436 ; RRID:Addgene_242436)

  • For your References section:

    Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system. Li F, Baranwal AK, Maerkl SJ. Nat Commun. 2025 Jul 5;16(1):6212. doi: 10.1038/s41467-025-61671-8. 10.1038/s41467-025-61671-8 PubMed 40617857
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